Eric Underbakke, BBMB Department, is the speaker. His presentation will take place in 1424 MBB at 4:10. Here is the title and abstract:
Architecture and activation of nitric oxide signaling effectors
The diatomic gas nitric oxide (NO) serves dual biological roles as both a signaling molecule and an inflammation agent. Nitric oxide synthase (NOS) is the biological source of NO, while soluble guanylate cyclase (sGC) is the primary receptor of nitric oxide (NO) signaling. NO binds the sGC heme cofactor stimulating synthesis of the second messenger cyclic-GMP (cGMP). As the central hub of NO/cGMP signaling pathways, NOS and sGC are important in diverse physiological processes such as vasodilation and neurotransmission. Both NOS and sGC are large, dynamic, multi-domain proteins, challenging targets for traditional structural biology approaches. As such, the higher-order architecture and regulatory mechanisms of NOS and sGC remain unclear. To define the architecture and activation mechanisms of the core components of NO signaling, we combined hydrogen/deuterium exchange mass spectrometry and single-particle electron microscopy. The hybrid approach conferred structural restraints to build structural models of NOS and sGC that inform on activation mechanisms.