Department of Plant Pathology and Microbiology
Unraveling developmental and immune networks using integrated omic profiling
Abstract: Integrated molecular atlases make possible systems biology approaches aimed at understanding biological phenomena. I will discuss the generation and analysis of a large-scale atlas quantifying mRNA levels, protein abundance and phosphorylation state in maize, which is unparalleled in its depth and scope. In addition to describing complex spatiotemporal patterns of gene activity, the atlas has enabled generation and interrogation of a number of different types of regulatory networks including mRNA and protein co-expression networks, a gene regulatory netork (GRN) and a novel kinase-substrate network. I will also present research examining the innate immune response of maize, to the highly virulent fungal pathogen, Cochliobolus carbonum. Specifically, this work focuses on how HC-toxin, a naturally-occuring histone dacetylase inhibitor, promotes pathogen virulence towards maize. These studies highlight the complex interplay of transcriptional, translational and post-translational events in dynamically remodeling the proteome.
Research in my lab investigates molecular mechanisms that underpin plant-microbe interactions. Our research focuses on immune signaling in corn and Arabidopsis. We specialize in mass spectrometry based proteomics to globally quantify protein abundance and post-translational modifications (including phosphorylation and acetylation). Using systems biology we integrate these data with other types of omics datasets, such as transcriptome profiling, to generate hypotheses to test using various biochemical and genetic approaches.
Area of Expertise:
- Plant-Microbe Interactions
- Systems Biology
- Plant Biology
- Genetics & Genomics
- Bioinformatics & Computational Biology
- Molecular, Cellular & Developmental Biology
- PhD, University of California - Davis, 2009
- MS, Miami University, 2005
- BS, University of Mount Union, 2001
- BCBIO 402. Fundamentals of Bioinformatics and Computational Biology II.
- PLP 692: Molecular Biology of Plant-Pathogen Interactions