Carla M Mann

People
Graduate Teaching Assistant
Dobbs Lab
Carla Mann

 

PAST RESEARCH EXPERIENCE:

Augustana College, Biology Department, Research Assistant, November 2012-present

  • Assisted in research on meiothermus ruber specifically related to identifying kanamycin resistance gene; tasked with solo project of confirming the existence of kanamycin resistance
  • Performed bioinformatic analysis using NCBI BLAST on M. ruber and found likely identity of previously identified hypothetical protein from Mrub_0554
  • Isolated and amplified Mrub_0554 gene and inserted into expression vector via ligase-independent cloning

QC Analytical Services, LeClaire, IA – Intern - January-August 2012

  • Performed water quality tests including water hardness, oil and grease, carbon content, sulfides, nitric and nitrous acid, volatile acids, biological oxygen demand, chemical oxygen demand, hexavalent chromium, total suspended solids, cyanide, ammonia, phenols, alkalinity
  • Co-led a project to examine the feasibility of expanding the business into feed testing

Augustana College, Biochemistry Department (spring 2011, summer 2012-present)

  • Researched the role of the zinc ion in amino peptidase-N's (pepN) catalytic efficiency.
  • Grew and over-expressed wild type and apo-pepN in E. coli.
  • Isolated proteins via dialysis and FPLC.
  • Performed enzyme kinetics on wild type and apo-pepN using L-alanine-P-nitroanilide as a substrate
  • Results were somewhat (but not significantly) consistent with literature theory; the results suggested the zinc ion is necessary for efficient catalytic function.
  • Performed ongoing research into antibiotic resistance involving β-lactams.
  • Transferred β-lactamase gene from pKT-1 plasmid to pET-26(b) plasmid over a six-week period.
  • Used restriction digest and gel electrophoresis to excise gene from pKT-1, and then used LIC to transfer the gene to pET-26(b).

Loyola University Medical Center, Department of Immunology and Microbiology, Intern, (2009)

  • worked under Dr. Katherine Knight's supervision on a project that determined the site of VDJ recombination in B-cells in mutant Alicia rabbits.
  • Used immunohistochemistry to identify and tag appendix tissue cells for analysis.
  • Dissected appendix tissue using laser capture and microdissection.
  • Cloned DNA from captured cells using PCR and then mass-replicated the antibody DNA via splicing into pGEM-T plasmid inserted into E. coli via electroporation.
  • Isolated DNA via WIZARD prep and sequenced the DNA in-house using Loyola's DNA sequencer.
  • Analyzed DNA sequences between VDJ regions in antibodies to determine localization of selection.
  • Determined selection occurs in the Ki67+ proliferating region of the appendix lumen.
Area of Expertise: 
functional prediction
genomics
Education: 
B.A., Biochemistry, Augustana College, 2013
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